Difference between revisions of "Part:BBa K2130001"
 
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<partinfo>Mutant Cas9 no. 462</partinfo>
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<partinfo>K2130001 short</partinfo>
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Cas9 mutant 462
  
 
This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.
 
This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.
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 +
Mutation sites:
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N869K and E904K
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Team Macquarie helped us made an in-vitro assay for the comparison of WT and mutant 459.
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https://static.igem.org/mediawiki/2016/f/f8/T--Macquarie_Australia--CollabSing_.png
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:37, 19 October 2016


Cas9 mutant 462

Cas9 mutant 462

This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.

Mutation sites: N869K and E904K

Team Macquarie helped us made an in-vitro assay for the comparison of WT and mutant 459. T--Macquarie_Australia--CollabSing_.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BglII site found at 1647
    Illegal BglII site found at 1736
    Illegal BglII site found at 2381
    Illegal BamHI site found at 4105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]