Difference between revisions of "Part:BBa K1985014"
Line 3: | Line 3: | ||
This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used. | This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used. | ||
− | + | ||
==Validation== | ==Validation== |
Revision as of 21:28, 19 October 2016
Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter
This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used.
Validation
The improved plasmid was validated in three ways:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961