Difference between revisions of "Part:BBa K1909013"
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<partinfo>BBa_K1909013 short</partinfo>
 
<partinfo>BBa_K1909013 short</partinfo>
 
Promoter (OmpR, positive) with GFP
 
Promoter (OmpR, positive) with GFP
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The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
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Usage and Biology
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The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
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In nature, the promoter OmpR is upstream of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. Via FACS analysis it was shown in EnvZ deficient cells (JW3367-3), containing the construct BBa_K1909013 ,that the expression of GFP was significantly reduced in comparison to EnvZ containing cells (DH5α).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
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In nature, the promoter OmpR is upstream of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. Via FACS analysis it was shown in EnvZ deficient cells (JW3367-3), containing the construct BBa_K1909013 ,that the expression of GFP was significantly reduced in comparison to EnvZ containing cells (DH5α).
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1909013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1909013 SequenceAndFeatures</partinfo>

Revision as of 21:04, 19 October 2016


Omp Promoter / GFP Promoter (OmpR, positive) with GFP

The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 779