Difference between revisions of "Part:BBa K1909013"

Revision as of 21:02, 19 October 2016

Omp Promoter / GFP Promoter (OmpR, positive) with GFP The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. Usage and Biology In nature, the promoter OmpR is upstream of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. Via FACS analysis it was shown in EnvZ deficient cells (JW3367-3), containing the construct BBa_K1909013 ,that the expression of GFP was significantly reduced in comparison to EnvZ containing cells (DH5α).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 779

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 __NOTOC__
 <partinfo>BBa_K1909013 short</partinfo>
+Promoter (OmpR, positive) with GFP
-Coming Soon
+The part BBa_K1909013 consists of the green fluorescent protein GFP and the positively regulated OmpR-controlled promoter (BBa_R0082). The promoter OmpR is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
+Usage and Biology
+In nature, the promoter OmpR is upstream of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. Via FACS analysis it was shown in EnvZ deficient cells (JW3367-3), containing the construct BBa_K1909013 ,that the expression of GFP was significantly reduced in comparison to EnvZ containing cells (DH5α).
 <!-- Add more about the biology of this part here