Difference between revisions of "Part:BBa K2150022"
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<partinfo>BBa_K2150022 short</partinfo>
 
<partinfo>BBa_K2150022 short</partinfo>
  
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In this part, the first promoter pTet is constitutively on, producing T7 RNA polymerase(T7RNAP). T7RNAP can activate the T7 promoter, thus producing GFP in large quantity.  
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In this part, T7 RNA polymerase(T7RNAP) is expressed from pTet which is inducible by tetracycline and its analogs. T7RNAP can activate the T7 promoter, thus producing GFP in large quantity.  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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We design this part to test whether the introduction of T7 polymerase and T7 promoter has the potential to enhance our scavenger's ability to degrade tetracycline in presence of tetR.
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===Characterization===
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The expression level of GFP rises as the concentration of aTc(Fig.1), in the presence of tetR, which indicates the DNA downstream of T7 promoter is inducible by tetracycline and its analogs.
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          [[File:Captain Simulator_1.png|250px]]
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Comparing to another design (see [https://parts.igem.org/Part:BBa_K2150014 BBa_K2150014])
  
 
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Revision as of 20:59, 19 October 2016


pTet+RBS+T7RNAP+Ter+pT7+RBS+GFP+DT

In this part, T7 RNA polymerase(T7RNAP) is expressed from pTet which is inducible by tetracycline and its analogs. T7RNAP can activate the T7 promoter, thus producing GFP in large quantity.


Usage and Biology

We design this part to test whether the introduction of T7 polymerase and T7 promoter has the potential to enhance our scavenger's ability to degrade tetracycline in presence of tetR.

Characterization

The expression level of GFP rises as the concentration of aTc(Fig.1), in the presence of tetR, which indicates the DNA downstream of T7 promoter is inducible by tetracycline and its analogs. 


          Captain Simulator 1.png

Comparing to another design (see BBa_K2150014)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3488