Difference between revisions of "Part:BBa K1985006"
 
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<partinfo>BBa_K1985006 short</partinfo>
 
<partinfo>BBa_K1985006 short</partinfo>
  
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MamO is involved in crystal nucleation.  
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MamO is a protein that is involved in nucleation of magnetite crystals.  
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[[File:PSB1C3-MamO snap pic.jpeg||400px|thumb|centre|The mamO construct in pSB1C3]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1985000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1985006 SequenceAndFeatures</partinfo>
  
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[[File:PSB1C3-MamO snap pic.jpeg||400px|thumb|left|Figure 1. The mamO construct in pSB1C3]]
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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Usage: The mamO gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to start the process that should form magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.  
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Usage: The mamO gene was used to initiate the formation of magnetite by "nucleating" the crystal particles, allowing further development. It was used in combination with the proposed electron transport complex of mamO, P and X in vivo to form magnetite. The part was used to assemble further composite parts [https://parts.igem.org/Part:BBa_K1985007 BBa_K1985007], [https://parts.igem.org/Part:BBa_K1985017 BBa_1985017], [https://parts.igem.org/Part:BBa_K1985008 BBa_K1985008] and [https://parts.igem.org/Part:BBa_K1985009 BBa_K1985009] in pSB1A3.  
  
 
Biology:
 
Biology:
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MamO has been described as a crystal nucleation protein. It is part of the magnetosome of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
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MamO is an integral membrane protein that with eight predicted transmembrane alpha helices. It has been described as a crystal nucleation protein, that may help initiate magnetite biomineralisation. Mutant studies show that M. gryphiswaldense cells with mutaions in the mamO gene are non-magnetitic. MamO is part of the magnetosome of the native species, Magnetospirillum gryphiswaldense. Together with the additional mam genes, it promotes magnetite crystal maturation within the magnetosome.
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===References===
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Murat, D., Quinlan, A., Vali, H., and Komeili, A. (2010). Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. Proc. Natl. Acad. Sci. U.S.A. 107, 5593–5598.
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Yang, W., Li, R., Peng, T., Zhang, Y., Jiang, W., Li, Y., et al. (2010). mamO and mamE genes are essential for magnetosome crystal biomineralization in Magnetospirillum gryphiswaldense MSR-1. Res. Microbiol. 161, 701–705.

Latest revision as of 20:41, 19 October 2016

mamO

MamO is a protein that is involved in nucleation of magnetite crystals.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 879
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 744
    Illegal AgeI site found at 828
    Illegal AgeI site found at 1737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1359
    Illegal BsaI.rc site found at 30
Figure 1. The mamO construct in pSB1C3


Usage and Biology

Usage: The mamO gene was used to initiate the formation of magnetite by "nucleating" the crystal particles, allowing further development. It was used in combination with the proposed electron transport complex of mamO, P and X in vivo to form magnetite. The part was used to assemble further composite parts BBa_K1985007, BBa_1985017, BBa_K1985008 and BBa_K1985009 in pSB1A3.

Biology: MamO is an integral membrane protein that with eight predicted transmembrane alpha helices. It has been described as a crystal nucleation protein, that may help initiate magnetite biomineralisation. Mutant studies show that M. gryphiswaldense cells with mutaions in the mamO gene are non-magnetitic. MamO is part of the magnetosome of the native species, Magnetospirillum gryphiswaldense. Together with the additional mam genes, it promotes magnetite crystal maturation within the magnetosome.

References

Murat, D., Quinlan, A., Vali, H., and Komeili, A. (2010). Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. Proc. Natl. Acad. Sci. U.S.A. 107, 5593–5598.

Yang, W., Li, R., Peng, T., Zhang, Y., Jiang, W., Li, Y., et al. (2010). mamO and mamE genes are essential for magnetosome crystal biomineralization in Magnetospirillum gryphiswaldense MSR-1. Res. Microbiol. 161, 701–705.