Difference between revisions of "Part:BBa K2088016"
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pbaA1A2B is responsible for the angular dioxygenation of 3-phenoxybenzoate(3-PBA). | pbaA1A2B is responsible for the angular dioxygenation of 3-phenoxybenzoate(3-PBA). | ||
Complementation of pbaA1A2B results in the active 3-phenoxybenzoate 1',2'-dioxygenase, but the enzyme activity in Escherichia coli was achieved only through the coexpression of pbaA1A2B and a glutathione reductase (GR)-type reductase gene, pbaC.[1] | Complementation of pbaA1A2B results in the active 3-phenoxybenzoate 1',2'-dioxygenase, but the enzyme activity in Escherichia coli was achieved only through the coexpression of pbaA1A2B and a glutathione reductase (GR)-type reductase gene, pbaC.[1] | ||
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In this part, pbaA1A2 and pbaB are under their native RBS. Besides, this pba gene cluster is followed by a double terminator B0015. | In this part, pbaA1A2 and pbaB are under their native RBS. Besides, this pba gene cluster is followed by a double terminator B0015. | ||
Latest revision as of 19:25, 19 October 2016
nativeRBS-pbaA1A2B with a double Terminator
pbaA1A2B is responsible for the angular dioxygenation of 3-phenoxybenzoate(3-PBA). Complementation of pbaA1A2B results in the active 3-phenoxybenzoate 1',2'-dioxygenase, but the enzyme activity in Escherichia coli was achieved only through the coexpression of pbaA1A2B and a glutathione reductase (GR)-type reductase gene, pbaC.[1]
In this part, pbaA1A2 and pbaB are under their native RBS. Besides, this pba gene cluster is followed by a double terminator B0015.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2648
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 73
Illegal NgoMIV site found at 116
Illegal NgoMIV site found at 776
Illegal NgoMIV site found at 1383
Illegal NgoMIV site found at 1407
Illegal AgeI site found at 1291 - 1000COMPATIBLE WITH RFC[1000]