Difference between revisions of "Part:BBa K2014000"
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− | | colspan = 2 | [[Image:https:// | + | | colspan = 2 | [[[Image:UAMpoznanaraall.jpg|800px|Link=https://static.igem.org/mediawiki/parts/8/80/BBa_K2014000-1.png]]|thumb|800px|center|<font size="1">Quantification of the fluorescence after 10h of growth</font>]] |
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Revision as of 19:22, 19 October 2016
pBAD-E15'UTR->sfGFP
pBAD-E15'UTR->sfGFP construct is derived from pBAD (Arashort1, BBa_K1741000) Escherichia coli K-12 arabinose promoter, which we fused with E1_5’UTR (Fig. 1). E1_5’UTR contains an additional ribosome binding site from gene 10 of bacteriophage T7. The new promoter controls the expression of sfGFP with a His-tag at its N-end. The fluorescent protein sfGFP is a marker of gene expression and protein synthesis/accumulation. Protein expression from all compared arabinose responsive promoters was induced in rich media with 0.4% L-arabinose.
[[[Image:UAMpoznanaraall.jpg|800px|Link=![]() |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 365