Difference between revisions of "Part:BBa K2014000"

Revision as of 19:22, 19 October 2016

pBAD-E15'UTR->sfGFP

pBAD-E15'UTR->sfGFP construct is derived from pBAD (Arashort1, BBa_K1741000) Escherichia coli K-12 arabinose promoter, which we fused with E1_5’UTR (Fig. 1). E1_5’UTR contains an additional ribosome binding site from gene 10 of bacteriophage T7. The new promoter controls the expression of sfGFP with a His-tag at its N-end. The fluorescent protein sfGFP is a marker of gene expression and protein synthesis/accumulation. Protein expression from all compared arabinose responsive promoters was induced in rich media with 0.4% L-arabinose.

[[[Image:UAMpoznanaraall.jpg|800px|Link=

]]|thumb|800px|center|Quantification of the fluorescence after 10h of growth]]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 71
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 53
Illegal SapI.rc site found at 365

@@ Line 6: / Line 6: @@
 {|align="center"
   |-valign="top"
-  | colspan = 2 | [[Image:https://parts.igem.org/File:BBa_K2014000-1.png|thumb|530px|center|<font size="1">Quantification of the fluorescence after 10h of growth</font>]]
+  | colspan = 2 | [[[Image:UAMpoznanaraall.jpg|800px|Link=https://static.igem.org/mediawiki/parts/8/80/BBa_K2014000-1.png]]|thumb|800px|center|<font size="1">Quantification of the fluorescence after 10h of growth</font>]]
 |}