Difference between revisions of "Part:BBa K2014000"
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<b>pBAD-E15'UTR->sfGFP</b> construct is derived from pBAD (Arashort1, BBa_K1741000) Escherichia coli K-12 arabinose promoter, which we fused with <b>E1_5’UTR</b> (Fig. 1). <b>E1_5’UTR</b> contains an additional ribosome binding site from gene 10 of bacteriophage T7. The new promoter controls the expression of sfGFP with a His-tag at its N-end. The fluorescent protein sfGFP is a marker of gene expression and protein synthesis/accumulation. Protein expression from all compared arabinose responsive promoters was induced in rich media with 0.4% L-arabinose. | <b>pBAD-E15'UTR->sfGFP</b> construct is derived from pBAD (Arashort1, BBa_K1741000) Escherichia coli K-12 arabinose promoter, which we fused with <b>E1_5’UTR</b> (Fig. 1). <b>E1_5’UTR</b> contains an additional ribosome binding site from gene 10 of bacteriophage T7. The new promoter controls the expression of sfGFP with a His-tag at its N-end. The fluorescent protein sfGFP is a marker of gene expression and protein synthesis/accumulation. Protein expression from all compared arabinose responsive promoters was induced in rich media with 0.4% L-arabinose. | ||
− | [[Image:https:// | + | {|align="center" |
+ | |-valign="top" | ||
+ | | colspan = 2 | [[Image:https://parts.igem.org/File:BBa_K2014000-1.png|thumb|530px|center|<font size="1">Quantification of the fluorescence after 10h of growth</font>]] | ||
+ | |} | ||
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Revision as of 19:08, 19 October 2016
pBAD-E15'UTR->sfGFP
pBAD-E15'UTR->sfGFP construct is derived from pBAD (Arashort1, BBa_K1741000) Escherichia coli K-12 arabinose promoter, which we fused with E1_5’UTR (Fig. 1). E1_5’UTR contains an additional ribosome binding site from gene 10 of bacteriophage T7. The new promoter controls the expression of sfGFP with a His-tag at its N-end. The fluorescent protein sfGFP is a marker of gene expression and protein synthesis/accumulation. Protein expression from all compared arabinose responsive promoters was induced in rich media with 0.4% L-arabinose.
File:Https://parts.igem.org/File:BBa K2014000-1.png Quantification of the fluorescence after 10h of growth |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 236
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 365