Difference between revisions of "Part:BBa K1943005:Experience"
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+ | ===Applications of BBa_K1943005=== | ||
+ | After we constructed this part, we transformed it into BL21 to test the expression efficiency. Meanwhile, we did a comparison with another part only whose promoter+RBS combination differs. | ||
− | + | ===Expression test of BBa_K1943005=== | |
− | + | We spread the plate for DH5α at 1:20am on 14th, Oct. The plate contains chloramphenicol. Following photo were taken every several hours after placing the plates into a 37℃ incubator. K1943005 is in the bottom-left corner in each photo. | |
− | + | ||
− | + | At 12:24 (14th,Oct.) | |
+ | [[File:6_7@1224.jpeg]] | ||
+ | |||
+ | At 14:20 (14th,Oct.) | ||
+ | [[File:6_7@1420.jpeg]] | ||
+ | |||
+ | At 18:30 (14th,Oct.) | ||
+ | [[File:6_7@1830.jpeg]] | ||
+ | |||
+ | At 20:21 (14th,Oct.) | ||
+ | [[File:6_7@2021.jpeg]] | ||
+ | |||
+ | At 22:40 (14th,Oct.) | ||
+ | [[File:6_7@2240.jpeg]] | ||
+ | |||
+ | At 8:50 (15th,Oct.) | ||
+ | [[File:6_7@850.jpeg]] | ||
+ | |||
+ | At 14:28 (15th,Oct.) | ||
+ | [[File:6_7@1428.jpeg]] | ||
===User Reviews=== | ===User Reviews=== |
Latest revision as of 18:35, 19 October 2016
Applications of BBa_K1943005
After we constructed this part, we transformed it into BL21 to test the expression efficiency. Meanwhile, we did a comparison with another part only whose promoter+RBS combination differs.
Expression test of BBa_K1943005
We spread the plate for DH5α at 1:20am on 14th, Oct. The plate contains chloramphenicol. Following photo were taken every several hours after placing the plates into a 37℃ incubator. K1943005 is in the bottom-left corner in each photo.
At 12:24 (14th,Oct.)
At 14:20 (14th,Oct.)
At 18:30 (14th,Oct.)
At 20:21 (14th,Oct.)
At 22:40 (14th,Oct.)
At 8:50 (15th,Oct.)
At 14:28 (15th,Oct.)
User Reviews
UNIQ65f806f1cb80ed49-partinfo-00000000-QINU UNIQ65f806f1cb80ed49-partinfo-00000001-QINU