Difference between revisions of "Part:BBa I20244:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I20244 short</partinfo> | <partinfo>BBa_I20244 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | . | + | Does not contain a full BB site upstream of the RBS to best simulate spacing in actual Promoter/RBS construction, which forms an Xba1/Spe1 mixed site. |
Latest revision as of 15:09, 15 August 2007
A device used to characterize promoters throught measurement of GFP expression
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Illegal prefix found in sequence at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 684
Design Notes
Does not contain a full BB site upstream of the RBS to best simulate spacing in actual Promoter/RBS construction, which forms an Xba1/Spe1 mixed site.
Source
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