Difference between revisions of "Part:BBa K2170050:Design"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa K2170050 short</partinfo>
+
<partinfo>BBa_K2170050 short</partinfo>
  
−
<partinfo>BBa K2170050 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2170050 SequenceAndFeatures</partinfo>
  
 
<br>'''Keywords:'''
 
<br>'''Keywords:'''
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
−
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
+
*Related BioBricks:
−
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170052 BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin]<br>
−
 
+
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K2170050 BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin]<br>
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
<!--*Designed in RFC10/RFC23/RFC25-->
+
*Designed in RFC10
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
−
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
+
 
  
 
'''Quality control measures:'''<br>
 
'''Quality control measures:'''<br>
−
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
+
*Test digestion using EcoRI & PstI
−
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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*Sequencing using primer VF2 & VR
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<!--*Part was partly sequenced/Part was totally sequenced-->
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*Part was partly sequenced/Part was totally sequenced
  
 
'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
<!--*Backbone name: pSB1C3'/?backbone_name?-->
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*Backbone name: pSB1C3
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<!--*Resistance: Amp/Cp/Kan/-->
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*Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
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*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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*Protein: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide [Nucleotide 1030 to 2652]-->
−
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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*Tag: internal A3C5 Tag
−
<!--*The protein encoded is posttranslationally modified by ???.-->
+
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
−
<!--none/EC-number ?.?.?.?-->
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* NanoLuc
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
<!--none/not known/cytotoxic for ''organism name''-->
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*none
  
 
'''Safety notes:'''<br>
 
'''Safety notes:'''<br>
−
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
+
Known and anticipated sefety issues: none
−
<!--Known and anticipated security issues: none/other.-->
+
Known and anticipated security issues: none
−
 
+
−
'''Intellectual property:'''
+
−
<!--Information on patent situation.-->
+
−
<!--Intellectual property claims made by the authors.-->
+
−
 
+
−
'''Corresponding part author/authors:'''
+
−
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
+
  
 
===Source===
 
===Source===
  
 
'''Source:'''<br>
 
'''Source:'''<br>
−
<!--*Commercial system: plasmid name, system name, company name-->
+
 
−
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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*Preexisting BioBrick BBa_I739001; BBa_B0015; BBa_K1159001
−
<!--*Preexisting BioBrick ?Bba_number?-->
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*Parts Synthesized by IDT
−
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
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*Professor Dr. Arne Skerra (Chair biological chemistry of TUM)
−
<!--*Synthesized by ?company_name?.-->
+
  
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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Genesequence derived from ''Escherichia coli''
 
Genesequence derived from ''Escherichia coli''
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
−
<!--*Designed for the following Chassis: ''?organism-name?''-->
+
*Designed for the following Chassis: procaryotic cells
 
<!--*Statement about functionality in other chassis.-->
 
<!--*Statement about functionality in other chassis.-->
  
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'''Literature references:'''<br>
 
'''Literature references:'''<br>
−
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
+
 
 +
1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]<br>
 +
2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]<br>
 +
3. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754705/ Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.]<br>
 +
4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]<br>
  
 
'''Database references:'''<br>
 
'''Database references:'''<br>

Latest revision as of 16:31, 19 October 2016


Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 889


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

  • Related BioBricks:

BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin
BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin
Cloning details:

  • Designed in RFC10


Quality control measures:

  • Test digestion using EcoRI & PstI
  • Sequencing using primer VF2 & VR
  • Part was partly sequenced/Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide [Nucleotide 1030 to 2652]-->
  • Tag: internal A3C5 Tag

Enzymatic activity:

  • NanoLuc

Cytotoxicity:

  • none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none

Source

Source:

  • Preexisting BioBrick BBa_I739001; BBa_B0015; BBa_K1159001
  • Parts Synthesized by IDT
  • Professor Dr. Arne Skerra (Chair biological chemistry of TUM)


Organism:
Genesequence derived from Escherichia coli

  • Designed for the following Chassis: procaryotic cells


References

Literature references:

1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]
3. Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.
4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]

Database references: