Difference between revisions of "Part:BBa K2036009"
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<partinfo>BBa_K2036009 parameters</partinfo>
 
<partinfo>BBa_K2036009 parameters</partinfo>
 
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<h2>Protein&promoter</h2>
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<p>--Cro and pRM</p>
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<br>
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[[File:T--HUST-China--CI-pR_inhibition.png|800px|thumb|center|Fig1: We characterized cro and pRM inhibition by the same method as CI and pR’s. From line chart and fluorescence detection, we can see that the test group contains cro expressed less GFP protein than control group over time. It proves that cro can effectively bind pRM to block its downstream gene’s transcription.]]
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<br>

Revision as of 16:27, 19 October 2016


pRM-GFP-LVAssrAtag

pRM-GFP-LVAtag, the circuit can function as a control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K20360010).

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 699


Protein&promoter

--Cro and pRM


Fig1: We characterized cro and pRM inhibition by the same method as CI and pR’s. From line chart and fluorescence detection, we can see that the test group contains cro expressed less GFP protein than control group over time. It proves that cro can effectively bind pRM to block its downstream gene’s transcription.