Difference between revisions of "Part:BBa K2114017"

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I) Surface Localization
 
I) Surface Localization
  
[[File:iG16_Freiburg_BBa_K2114017 GFP staining.png|350px|thumb|left|Figure 1: Schematic representation of the fusion protein.]]
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[[File:iG16_Freiburg_Alexa_FACS_BBa_K2114017.png|350px|thumb|left|Figure 2:   .]]
  
 
<br><br><br><br><br><br>
 
<br><br><br><br><br><br>
 
<br><br><br><br><br><br>
 
<br><br><br><br><br><br>
II) GFP binding
 
  
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II) GFP binding
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[[File:iG16_Freiburg_BBa_K2114017 GFP staining.png|350px|thumb|left|Figure 3:  .]]
  
 
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Revision as of 16:12, 19 October 2016


aGFPnano_HA_aHelix_cgeA

N-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by an alpha helical linker.


Usage and Biology

Figure 1: Schematic representation of the fusion protein.














Characterization

I) Surface Localization

Figure 2: .














II) GFP binding

Figure 3: .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]