Difference between revisions of "Part:BBa K2117005"

 
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<partinfo>BBa_K2117005 short</partinfo>
 
<partinfo>BBa_K2117005 short</partinfo>
  
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This is a composite part consisting of the TEF1 promoter (BBa_K2117000) and hrGFP (BBa_K2117003).
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This part encodes the humanized Renilla reniformis green fluorescent protein (hrGFP) codon-optimized for <i>Yarrowia lipolytica</i> and the native <i>Y. lipolytica</i> constitutive promoter TEF1.
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Studies have shown the hrGFP to be functional in  <i>Y. lipolytica</i> under the expression of the TEF1 promoter measured by flow cytometry. *insert reference*
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The green fluorescent protein originates from the sea pansy, <i>Renilla reniformis</i>.
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This part can be used as a reporter protein for protein expression in the yeast chassis, <i>Y. lipolytica</i>. It is compatible with all the iGEM Biobrick standards.
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Emission: 500-600 nm
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<p></p>
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Excitation: 488 nm
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<p></p>
  
 
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<!-- Uncomment this to enable Functional Parameter display
 
 
===Functional Parameters===
 
===Functional Parameters===
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Emission: 500-600 nm
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<p></p>
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Excitation: 488 nm
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<p></p>
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<partinfo>BBa_K2117005 parameters</partinfo>
 
<partinfo>BBa_K2117005 parameters</partinfo>
 
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Revision as of 16:04, 19 October 2016


Device encoding TEF1 and hrGFP for expression in Yarrowia lipolytica

This is a composite part consisting of the TEF1 promoter (BBa_K2117000) and hrGFP (BBa_K2117003).

This part encodes the humanized Renilla reniformis green fluorescent protein (hrGFP) codon-optimized for Yarrowia lipolytica and the native Y. lipolytica constitutive promoter TEF1.

Studies have shown the hrGFP to be functional in Y. lipolytica under the expression of the TEF1 promoter measured by flow cytometry. *insert reference*


Usage and Biology

The green fluorescent protein originates from the sea pansy, Renilla reniformis. This part can be used as a reporter protein for protein expression in the yeast chassis, Y. lipolytica. It is compatible with all the iGEM Biobrick standards.

Emission: 500-600 nm

Excitation: 488 nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Functional Parameters

Emission: 500-600 nm

Excitation: 488 nm