Difference between revisions of "Part:BBa K1959003"

Revision as of 16:03, 19 October 2016

BKT coding sequence fused with Pea transit peptide

This part contains the coding sequence (CDS) of β-carotene ketolase (BKT, EC 1.14.11.B16) of algae (Chlamydomonas reinhardti), which catalyzes the conversion of zeaxanthin to astaxanthin. A Pea transit peptide of RUBISCO small subunit has been fused to BKT and the codon has been optimized for rice (Oryza sativa).

Usage and Biology

　As a strong antioxidant, astaxanthin synthesis, especially, its biosynthesis attracts much interest of scientists. BKT symmetrically introduces two keto groups to two β-ionone rings of zeaxanthin to generate astaxanthin in algae (Figure.1).
　In our reconstructed pathway, the CDS of BKY was codon-optimized for rice. In addition, a Pea transit peptide was fused to the BKY for proper sorting into the plastid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1085
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 478
Illegal SapI site found at 900

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1959003 short</partinfo>
-This part is consist of a <em>Pea</em> transit peptide and the coding sequence of β-carotene ketolase originally derived from
+This part contains the coding sequence (CDS) of β-carotene ketolase (BKT, EC 1.14.11.B16) of algae (''Chlamydomonas reinhardti''), which catalyzes the conversion of zeaxanthin to astaxanthin. A ''Pea'' transit peptide of RUBISCO small subunit has been fused to BKT and the codon has been optimized for rice (''Oryza sativa'').
-<em>Chlamydomonas reinhardtii</em>. β-carotene ketolase realizes the conversion of zeaxanthin to astaxanthin, the final step of astaxanthin biosynthesis. Sequence of β-carotene ketolase has been optimized for better expression in rice (<em>Oryza sativa</em>).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+　As a strong antioxidant, astaxanthin synthesis, especially, its biosynthesis attracts much interest of scientists. BKT symmetrically introduces two keto groups to two β-ionone rings of zeaxanthin to generate astaxanthin in algae (Figure.1).<br>　In our reconstructed pathway, the CDS of ''BKY'' was codon-optimized for rice. In addition, a ''Pea'' transit peptide was fused to the BKY for proper sorting into the plastid.
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