Difference between revisions of "Part:BBa K2114017"
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===Characterization===
 
===Characterization===
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I) Surface Localization
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[[File:iG16_Freiburg_BBa_K2114017 GFP staining.png|350px|thumb|left|Figure 1: Schematic representation of the fusion protein.]]
 
[[File:iG16_Freiburg_BBa_K2114017 GFP staining.png|350px|thumb|left|Figure 1: Schematic representation of the fusion protein.]]
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<br><br><br><br><br><br>
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<br><br><br><br><br><br>
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II) GFP binding
  
  

Revision as of 15:30, 19 October 2016


aGFPnano_HA_aHelix_cgeA

N-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by an alpha helical linker.


Usage and Biology

Figure 1: Schematic representation of the fusion protein.

Characterization

I) Surface Localization

Figure 1: Schematic representation of the fusion protein.













II) GFP binding


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]