Difference between revisions of "Part:BBa K2036006"
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<partinfo>BBa_K2036006 parameters</partinfo>
 
<partinfo>BBa_K2036006 parameters</partinfo>
 
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<h1>Protein&protein reaction</h1>
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We had submitted and documentedRBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were to test whether CIII can protect CII from being degraded by Ftshby competitive inhibition.
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[[File:T--HUST-China--CIII%26Ftsh.png|800px|thumb|center|Figure 1: According to the Flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increasedover time and it showed significant difference from two control groups. It indicates that tandomly expressed CIII can efficiently protect CII from being degraded by Ftsh.]]

Revision as of 15:12, 19 October 2016


RBS-CIII

CIII ( BBa_K2036001 )is a inhibitor of Ftsh,we add a RBS to provide more possibilities to users to create more complex circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Protein&protein reaction

We had submitted and documentedRBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036014) and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015). These two parts were to test whether CIII can protect CII from being degraded by Ftshby competitive inhibition.


Figure 1: According to the Flourescence measurement curve above, we can see clearly that GFP level of CIII test circuit increasedover time and it showed significant difference from two control groups. It indicates that tandomly expressed CIII can efficiently protect CII from being degraded by Ftsh.