Difference between revisions of "Part:BBa K1896015"
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<partinfo>BBa_K1896015 short</partinfo> | <partinfo>BBa_K1896015 short</partinfo> | ||
| − | + | This part constitutively expresses a fusion protein comprising monomeric variants of GFPuv ([[Part:BBa_K1896001|mGFPuv2]]) and Streptavidin ([[Part:BBa_K1896000|mSA2]]). When the crude lysate of a culture of ''E. coli'' that contains this part is spread over solid biotinylated poly lactic acid (PLA), the strong affinity of mSA2 causes the fusion protein to adhere to the PLA, resisting washing with saline. | |
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| + | +++ Photo of attachment to biotin-PLA coated glass slides+++ | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:43, 19 October 2016
mGFPuv2-mSA2 generator
This part constitutively expresses a fusion protein comprising monomeric variants of GFPuv (mGFPuv2) and Streptavidin (mSA2). When the crude lysate of a culture of E. coli that contains this part is spread over solid biotinylated poly lactic acid (PLA), the strong affinity of mSA2 causes the fusion protein to adhere to the PLA, resisting washing with saline.
+++ Photo of attachment to biotin-PLA coated glass slides+++
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 872
Illegal AgeI site found at 932 - 1000COMPATIBLE WITH RFC[1000]