Difference between revisions of "Part:BBa K2172009"

Revision as of 13:14, 19 October 2016

Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator

This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin protease cleavage site plus a TEV restriction enzyme cleavage site and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not. Other parts, such as the gene SmCPS1, can be inserted into this part via digestion on the thrombin site and the TEV site. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 747
Illegal XhoI site found at 1485
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 159

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2172009 short</partinfo>
-This part can be used to make other parts that can be purified, cleaved and detected for expression conveniently. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin protease cleavage site and a GFP detection unit.
+This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin protease cleavage site plus a TEV restriction enzyme cleavage site and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not. Other parts, such as the gene SmCPS1, can be inserted into this part via digestion on the thrombin site and the TEV site.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===