Difference between revisions of "Part:BBa K2170001:Design"

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'''Literature references:'''<br>
 
'''Literature references:'''<br>
 
[http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html >Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]
 
[http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html >Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]
 +
[http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
 +
[http://nar.oxfordjournals.org/content/15/20/8125.short Kozak, M. (1987). An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic acids research, 15(20), 8125-8148.]
 +
[http://www.nature.com/nmeth/journal/v8/n10/abs/nmeth.1701.html Petersen, T. N., Brunak, S., von Heijne, G., & Nielsen, H. (2011). SignalP 4.0: discriminating signal peptides from transmembrane regions. Nature methods, 8(10), 785-786.]
  
 
'''Database references:'''<br>
 
'''Database references:'''<br>

Revision as of 13:09, 19 October 2016


Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2432


Design Notes

Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA

Cloning details:
Designed in RFC25

Quality control measures:
Test digestion using EcorRI & PstI Sequencing using VF2 Part was partly sequenced

Backbone:
Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:
Protein: Secretory eukaryotic biotin binding receptor with single chain avidin [Nucleotide 633 to 3314]


Enzymatic activity: none

Cytotoxicity:
not known

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none

Intellectual property:

Corresponding part author/authors:

Source

Source:
Parts synthesized by IDT


Organism:
Genesequence derived from Escherichia coli


References

Literature references:
[http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html >Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.] [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.] [http://nar.oxfordjournals.org/content/15/20/8125.short Kozak, M. (1987). An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic acids research, 15(20), 8125-8148.] [http://www.nature.com/nmeth/journal/v8/n10/abs/nmeth.1701.html Petersen, T. N., Brunak, S., von Heijne, G., & Nielsen, H. (2011). SignalP 4.0: discriminating signal peptides from transmembrane regions. Nature methods, 8(10), 785-786.]

Database references: