Difference between revisions of "Part:BBa K1930006:Design"
Barawaclawik (Talk | contribs) |
Barawaclawik (Talk | contribs) |
||
Line 20: | Line 20: | ||
<html> | <html> | ||
<p> See <a href="http://2016.igem.org/Team:Groningen/Decoy">Decoy experiment</a>.</p> | <p> See <a href="http://2016.igem.org/Team:Groningen/Decoy">Decoy experiment</a>.</p> | ||
− | <p> [1]<a href="http://aem.asm.org/content/79/20/6481.full">Overkamp et al. 2013</a> - Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging.</p> | + | <p> [1] <a href="http://aem.asm.org/content/79/20/6481.full">Overkamp et al. 2013</a> - Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging.</p> |
</html> | </html> |
Latest revision as of 13:04, 19 October 2016
sfGFP(Sp)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
sfGFP(Sp) originally optimized for Streptococcus pneumoniae. It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs).
Source
Plasmid pNW
References
See Decoy experiment.
[1] Overkamp et al. 2013 - Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging.