Difference between revisions of "Part:BBa K1930006:Design"

 
 
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===Design Notes===
 
===Design Notes===
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sfGFP originally optimized for streptococcus pneumonia because it could be shown that the signal in Bacillus is even brighter than the one from sfGFP(Bs).
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<p>sfGFP(Sp) originally optimized for <em>Streptococcus pneumoniae</em>. It has been shown when expressed in <em>B. subtilis</em> that the signal is even brighter than the one from sfGFP(Bs).</p>
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===References===
 
===References===
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<p> See <a href="http://2016.igem.org/Team:Groningen/Decoy">Decoy experiment</a>.</p>
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<p> [1] <a href="http://aem.asm.org/content/79/20/6481.full">Overkamp et al. 2013</a> - Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging.</p>
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Latest revision as of 13:04, 19 October 2016


sfGFP(Sp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sfGFP(Sp) originally optimized for Streptococcus pneumoniae. It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs).


Source

Plasmid pNW

References

See Decoy experiment.

[1] Overkamp et al. 2013 - Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging.