Difference between revisions of "Part:BBa K2170001:Design"
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'''Cloning details:'''<br> | '''Cloning details:'''<br> | ||
Designed in RFC25 | Designed in RFC25 | ||
| − | Mutation C889G to delete XbaI restriction site | + | <!--Mutation C889G to delete XbaI restriction site--> |
| − | Truncation upstream/downstream compared to template, ?explanation? | + | <!--Truncation upstream/downstream compared to template, ?explanation?--> |
'''Quality control measures:'''<br> | '''Quality control measures:'''<br> | ||
| Line 25: | Line 25: | ||
Backbone name: pSB1C3 | Backbone name: pSB1C3 | ||
Resistance: Cp | Resistance: Cp | ||
| − | + | Copynumber: high | |
'''Protein coding:'''<br> | '''Protein coding:'''<br> | ||
Revision as of 13:02, 19 October 2016
Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2432
Design Notes
Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA
Cloning details:
Designed in RFC25
Quality control measures:
Test digestion using EcorRI & PstI
Sequencing using VF2
Part was partly sequenced
Backbone:
Backbone name: pSB1C3
Resistance: Cp
Copynumber: high
Protein coding:
Protein: Secretory eukaryotic biotin binding receptor with single chain avidin [Nucleotide 633 to 3314]
Enzymatic activity: none
Cytotoxicity:
not known
Safety notes:
Known and anticipated sefety issues: none
Corresponding part author/authors:
Source
Source:
Parts synthesized by IDT
Organism:
Genesequence derived from Escherichia coli
References
Literature references:
Database references: