Difference between revisions of "Part:BBa K1930006"
EikeMahlandt (Talk | contribs) (→Usage and Biology) |
Barawaclawik (Talk | contribs) |
||
(One intermediate revision by one other user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K1930006 short</partinfo> | <partinfo>BBa_K1930006 short</partinfo> | ||
− | sfGFP(Sp) is a reporter gene originally optimized for <em>Streptococcus pneumoniae</em>. | + | <html> |
+ | <style> | ||
+ | .wrap{ | ||
+ | position: relative; | ||
+ | } | ||
+ | img{ | ||
+ | max-width: 99%; | ||
+ | } | ||
+ | .split{ | ||
+ | position: relative; | ||
+ | display: flex; | ||
+ | flex-flow: row nowrap; | ||
+ | justify-content: space-between; | ||
+ | } | ||
+ | .split > .left{ | ||
+ | order: 1; | ||
+ | margin-right: 3px; | ||
+ | } | ||
+ | .split > .right{ | ||
+ | order: 2; | ||
+ | margin-left: 3px; | ||
+ | } | ||
+ | .split > .flone{ | ||
+ | flex: 1 1; | ||
+ | position: relative; | ||
+ | } | ||
+ | .split > .fltwo{ | ||
+ | flex: 2 1; | ||
+ | position: relative; | ||
+ | } | ||
+ | </style> | ||
+ | <p> sfGFP(Sp) is a reporter gene originally optimized for <em>Streptococcus pneumoniae</em>. </p> | ||
− | It has been shown when expressed in <em>B. subtilis</em> that the signal is even brighter than the one from sfGFP(Bs). | + | <p>It has been shown when expressed in <em>B. subtilis</em> that the signal is even brighter than the one from sfGFP(Bs). <a href="http://aem.asm.org/content/79/20/6481.full">[1]</a>.</p> |
+ | |||
+ | <figure> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/6/62/T-Groningen-pSB1C3-sfGFP.png"> | ||
+ | </figure> | ||
+ | </html> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <html> | ||
+ | <style> | ||
+ | .wrap{ | ||
+ | position: relative; | ||
+ | } | ||
+ | img{ | ||
+ | max-width: 99%; | ||
+ | } | ||
+ | .split{ | ||
+ | position: relative; | ||
+ | display: flex; | ||
+ | flex-flow: row nowrap; | ||
+ | justify-content: space-between; | ||
+ | } | ||
+ | .split > .left{ | ||
+ | order: 1; | ||
+ | margin-right: 3px; | ||
+ | } | ||
+ | .split > .right{ | ||
+ | order: 2; | ||
+ | margin-left: 3px; | ||
+ | } | ||
+ | .split > .flone{ | ||
+ | flex: 1 1; | ||
+ | position: relative; | ||
+ | } | ||
+ | .split > .fltwo{ | ||
+ | flex: 2 1; | ||
+ | position: relative; | ||
+ | } | ||
+ | </style> | ||
+ | <p><em>E.coli</em> appears green, when grown with this BioBrick. </p> | ||
+ | |||
+ | <figure> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/b/b0/T--Groningen--sfgfp-plate.jpeg"> | ||
+ | |||
+ | <figcaption>Figure 1. sfGFP(Sp) in <em>E.coli</em> Top10.</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <p> When the sfGFP is integrated into the genome of <em>B. subtilis</em> 168 the colonies do not appear green by eye but under the microscope the GFP signal is well seen.</p> | ||
+ | |||
+ | <figure> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/6/61/T--Groningen--sfgfp-microscopy.jpeg"> | ||
− | < | + | <figcaption>Figure 2. sfGFP(Sp) in <em>B. subtilis</em> 168.</figcaption> |
+ | </figure> | ||
− | < | + | <p>Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.</p> |
− | Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis. | + | </html> |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 12:59, 19 October 2016
sfGFP(Sp)
sfGFP(Sp) is a reporter gene originally optimized for Streptococcus pneumoniae.
It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs). [1].
![](https://static.igem.org/mediawiki/parts/6/62/T-Groningen-pSB1C3-sfGFP.png)
Usage and Biology
E.coli appears green, when grown with this BioBrick.
![](https://static.igem.org/mediawiki/parts/b/b0/T--Groningen--sfgfp-plate.jpeg)
When the sfGFP is integrated into the genome of B. subtilis 168 the colonies do not appear green by eye but under the microscope the GFP signal is well seen.
![](https://static.igem.org/mediawiki/parts/6/61/T--Groningen--sfgfp-microscopy.jpeg)
Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]