Difference between revisions of "Part:BBa K1930005:Design"

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===Design Notes===
 
===Design Notes===
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The promoter P<sub>AtpI</sub> is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.
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<p> The promoter P<sub>AtpI</sub> is a constitutive promoter which has its origin in <em>Bacillus subtilis</em>. It is responsible for the expression of <em>atpA</em> gene (ATP synthesis) during the first 30 minutes of the germination of <em>B. subtilis</em>. <em>atpA</em> gene is part of an operon (<em>atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC</em>), therefore the promoter region in front of the first protein coding gene (<em>atpI</em>) in this operon was chosen.</p>
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The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter P<sub>AtpI</sub> in the pSB1C3 backbone to make it available to other iGEM teams. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments
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<p>For more information see plasmid construction under our lab journal: (<a href="http://2016.igem.org/Team:Groningen/Labjournal#PatpI-in-pSB1C3">BBa_K1930005</a>.)</p>
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===Source===
 
===Source===
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===References===
 
===References===
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<p>[https://www.ncbi.nlm.nih.gov/pubmed/25661487 Sinai ''et al.'']</p>
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<p> <a href="https://www.ncbi.nlm.nih.gov/pubmed/25661487">Sinai et al. 2015</a> </p>
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<p>[http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA Subtiwiki-atpA]</p>
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<p><a href="http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA">Subtiwiki-atpA</a></p>

Latest revision as of 12:47, 19 October 2016


PAtpI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter PAtpI is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

For more information see plasmid construction under our lab journal: (BBa_K1930005.)

Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Sinai et al. 2015

Subtiwiki-atpA