Difference between revisions of "Part:BBa K2030004:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The complete sequence of <i>Saccharomyces cerevisiae</i> S288c chromosome XIV (Genbank accession number BK006947) was used to design primers for amplification of this promoter.  
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This part was not submitted due to illegal restriction sites.
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===Source===
 
===Source===

Revision as of 12:41, 19 October 2016


pGLN1 S. cerevisiae promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 175
    Illegal BamHI site found at 735
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was not submitted due to illegal restriction sites.

Source

Genomic DNA from Saccharomyces cerevisiae CEN.PK113-5D.

References