Difference between revisions of "Part:BBa K1934061:Experience"
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===Applications of BBa_K1934061=== | ===Applications of BBa_K1934061=== | ||
| − | + | This part has been transferred into <i>E. coli</i> NM522 for expression. After 6 hours at 37°C, IPTG was added to a final concentration of 1mM. The expression culture was grown overnight at 30°C. We then used instructions provided on the Ni-NTA columns kit from Qiagen to purify the protein. | |
===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 11:55, 19 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1934061
This part has been transferred into E. coli NM522 for expression. After 6 hours at 37°C, IPTG was added to a final concentration of 1mM. The expression culture was grown overnight at 30°C. We then used instructions provided on the Ni-NTA columns kit from Qiagen to purify the protein.
User Reviews
UNIQb3c3f21477af1ee0-partinfo-00000000-QINU UNIQb3c3f21477af1ee0-partinfo-00000001-QINU