Difference between revisions of "Part:BBa K1955009"

 
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<partinfo>BBa_K1955009 short</partinfo>
 
<partinfo>BBa_K1955009 short</partinfo>
  
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In order to prove the OVA sequence which we designed can smoothly express protein, we use BL21 competent cell to express the OVA sequence and detect the protein by Western blot analysis.
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In order to prove the OVA(Ovalbumin) sequence which we designed can express protein correctly, we use BL21 competent cell to express the HA/OVA sequence and detect the protein by Western blot analysis.
  
 
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Revision as of 11:47, 19 October 2016


pSB1C3-LacI inducible OVA(Ovalbumin)

In order to prove the OVA(Ovalbumin) sequence which we designed can express protein correctly, we use BL21 competent cell to express the HA/OVA sequence and detect the protein by Western blot analysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 232
    Illegal BamHI site found at 2336
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1463