Difference between revisions of "Part:BBa K1941005:Design"

 
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
some considerations!
+
The overall structure of the scRNA with MS2 hairpin was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting CYC1 in the c3 region of the promoter, as described in "Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas", F. Farzadfard et al., ASC Synth Biol. 2013.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 11:42, 19 October 2016


scCyc1_MS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
    Illegal NgoMIV site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The overall structure of the scRNA with MS2 hairpin was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting CYC1 in the c3 region of the promoter, as described in "Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas", F. Farzadfard et al., ASC Synth Biol. 2013.

Source

some source!

References