Difference between revisions of "Part:BBa B0034"

(Usage and Biology)
(Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
William and Mary iGEM 2016
 
 
https://static.igem.org/mediawiki/2016/5/58/T--William_and_Mary--RBS_Characterization_Curves.png
 
 
We characterized the Anderson RBS library to better understand the strengths of each RBS under different induction conditions
 
  
 
== IIT Madras 2016's Characterization ==
 
== IIT Madras 2016's Characterization ==

Revision as of 09:16, 19 October 2016

RBS (Elowitz 1999) -- defines RBS efficiency

RBS based on Elowitz repressilator.

Usage and Biology

IIT Madras 2016's Characterization

Characterization of popular BioBrick RBSs

Modelling

Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]

Experimentation

Biobrick RBSs B0030, B0031, B0032, B0034 were used in our 'Noise in Device' experiment to understand the role of RBS parts in giving rise to noise.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

biology-NA-
efficiency1

Note: The Elowitz RBS is the definition of efficiency 1.0.


Team Warsaw 2010's measurement

definition of 100% strength in our measurement.