Difference between revisions of "Part:BBa K1926022:Design"
 
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The SNAP-tag® and mCherry with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly.   
 
The SNAP-tag® and mCherry with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly.   
  
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[[File:5-连接过程改.png|800px|thumb|left|'''Figure 2:''' Three steps to construct our UNITs using PCR and oligonucleotide ligation.]]
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[[File:5-连接过程改.png|600px|thumb|left|'''Figure 2:''' Three steps to construct our UNITs using PCR and oligonucleotide ligation.]]
 
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Latest revision as of 07:34, 19 October 2016

The SNAP-mCHERRY UNIT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SNAP-tag® and mCherry with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly.

Figure 2: Three steps to construct our UNITs using PCR and oligonucleotide ligation.


Source

The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The sequence of Vox and DBOX was retrieved from Addgene and got through denovo synthesis. mCHERRY was got from commercial plasmid: pentry dcas9 mcherry bira.