Difference between revisions of "Part:BBa K1926021:Design"

 
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<partinfo>BBa_K1926021 SequenceAndFeatures</partinfo>
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===Design Notes===
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The SNAP-tag® and VIKA with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly. 
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[[File:5-连接过程改.png|600px|thumb|left|'''Figure 2:''' Three steps to construct our UNITs using PCR and oligonucleotide ligation.]]
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===Source===
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The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The recombinase gene VIKA and its RTS Vox were got through denovo synthesis.

Latest revision as of 07:33, 19 October 2016

The SNAP-VIKA UNIT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SNAP-tag® and VIKA with tags and RTSs are constructed using PCR and oligonucleotide ligation by the following procedure. Our UNITs all contain XbaI and SpeI enzyme sites, so we can easily line up the UNITs using 3A assembly.

Figure 2: Three steps to construct our UNITs using PCR and oligonucleotide ligation.


Source

The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The recombinase gene VIKA and its RTS Vox were got through denovo synthesis.