Difference between revisions of "Part:BBa K1926003:Design"

 
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===Design Notes===
 
===Design Notes===
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None
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We got the sequence from human genome through PCR using the following primers:
  
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===Source===
 
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The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:
 
 
pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC
 
pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC
 +
 
pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA
 
pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA
 +
 
(Product: 457)
 
(Product: 457)
  
 +
 +
The point mutation C→A was done at 284 to remove PstⅠ restriction site.
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 +
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Details are all at the [https://parts.igem.org/Part:BBa_K1926001 main page].
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 +
===Source===
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 +
The sequence was retrieved from Addgene.
  
 
===References===
 
===References===
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1. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76.

Latest revision as of 07:17, 19 October 2016


A cyclic promoter of CDK4 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 522
    Illegal NheI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
    Illegal AgeI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got the sequence from human genome through PCR using the following primers:

pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC

pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA

(Product: 457)


The point mutation C→A was done at 284 to remove PstⅠ restriction site.


Details are all at the main page.

Source

The sequence was retrieved from Addgene.

References

1. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76.