Difference between revisions of "Part:BBa K2172006"

 
 
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<partinfo>BBa_K2172006 short</partinfo>
 
<partinfo>BBa_K2172006 short</partinfo>
  
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This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site (for adding other segments) and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not.
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This gene codes for the enzyme copalyl diphosphate synthase 1(SmCPS1) in the plant Salvia miltiorrhiza. The enzyme is a part of the biosynthesis pathway of Tanshinone, a compound used to treat cardiovascular diseases.
  
 
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Latest revision as of 07:06, 19 October 2016


-SmCPS1-

This gene codes for the enzyme copalyl diphosphate synthase 1(SmCPS1) in the plant Salvia miltiorrhiza. The enzyme is a part of the biosynthesis pathway of Tanshinone, a compound used to treat cardiovascular diseases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1896
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 657
    Illegal BamHI site found at 255
    Illegal XhoI site found at 391
    Illegal XhoI site found at 415
    Illegal XhoI site found at 1708
    Illegal XhoI site found at 1918
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 60
    Illegal NgoMIV site found at 1477
    Illegal NgoMIV site found at 2041
  • 1000
    COMPATIBLE WITH RFC[1000]