Difference between revisions of "Part:BBa K2100036"
Line 3: | Line 3: | ||
<partinfo>BBa_K2100036 short</partinfo> | <partinfo>BBa_K2100036 short</partinfo> | ||
− | This | + | This construct is an expression vector containing the TRE promoter upstream of the BM3R1 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the BM3R1 repressor. BM3R1 represses the DNA sequences found on the pBM3R1 promoter with the help of the corepressor VP16-Gal4. |
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 05:56, 19 October 2016
pEXPR TRE-BM3R1
This construct is an expression vector containing the TRE promoter upstream of the BM3R1 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the BM3R1 repressor. BM3R1 represses the DNA sequences found on the pBM3R1 promoter with the help of the corepressor VP16-Gal4.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal XbaI site found at 30 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal NheI site found at 453
Illegal NotI site found at 342 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal XbaI site found at 30 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 580
Illegal XbaI site found at 30 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 446
Illegal BsaI site found at 573