Difference between revisions of "Part:BBa I718002:Experience"
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===Applications of BBa_I718002=== | ===Applications of BBa_I718002=== | ||
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| + | We transformed DGAT gene in pBluescript SK minus vector (Stratagene) (ampicilline resistance and pLac promoter) into E.coli chemically competent (DH5alpha). We used Nile Red fluorescence dye in the medium (5µg/mL medium) to observe lipid inclusions in different conditions (0.4mM IPTG induction in LB medium with or without sodium oleate 2mM). | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:29, 6 August 2007
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Applications of BBa_I718002
We transformed DGAT gene in pBluescript SK minus vector (Stratagene) (ampicilline resistance and pLac promoter) into E.coli chemically competent (DH5alpha). We used Nile Red fluorescence dye in the medium (5µg/mL medium) to observe lipid inclusions in different conditions (0.4mM IPTG induction in LB medium with or without sodium oleate 2mM).
User Reviews
UNIQ2caf0e5c32caaa73-partinfo-00000000-QINU UNIQ2caf0e5c32caaa73-partinfo-00000001-QINU