Difference between revisions of "Part:BBa K2130001"
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__NOTOC__
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Cas9 mutant 462
 
Cas9 mutant 462

Revision as of 04:48, 19 October 2016


BBa_K2130001

Cas9 mutant 462

This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BglII site found at 1647
    Illegal BglII site found at 1736
    Illegal BglII site found at 2381
    Illegal BamHI site found at 4105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]