Difference between revisions of "Part:BBa K799027"
 
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<partinfo>BBa_K799027 short</partinfo>
 
<partinfo>BBa_K799027 short</partinfo>
  
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CYP2E1 gene encodes the protein, Cytochrome P450 2E1 protein.It possesses a few functions such as its participation in the oxidative metabolism of a range of substrates like small polar molecules. Furthermore, it is able to convert ethanol to acetaldehyde and acetate in humans.
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CYP2E1 gene encodes the protein, Cytochrome P450 2E1 protein. It possesses a few functions such as its participation in the oxidative metabolism of a range of substrates like small polar molecules. Furthermore, it efficiently converts ethanol into acetaldehyde.
  
 
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[[Image:CYP2E1 sequencing.png]]
 
[[Image:CYP2E1 sequencing.png]]
  
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This is the sequencing result for the CYP2E1 human codon optimized gene. As can be seen, it is a perfect match to the expected sequence.
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This is the sequencing result for the CYP2E1 human gene, codon optimized for S. cerevisiae expression. As can be seen, it is a perfect match to the expected sequence.
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===Application===
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CCA_SanDiego:
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We successfully incorporated the CYP2e1 gene alongside the required NADPH P450 reductase gene in a bicistronic construct for coexpression in bacteria. The aim is to use this construct for bioremediation purposes through expression in E. Coli.
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[[Image:T--CCA_SanDiego--CYP2e1_Application.png]]

Latest revision as of 03:01, 19 October 2016

CYP2E1

CYP2E1 gene encodes the protein, Cytochrome P450 2E1 protein. It possesses a few functions such as its participation in the oxidative metabolism of a range of substrates like small polar molecules. Furthermore, it efficiently converts ethanol into acetaldehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1496


Plasmid Map

CYP2E1 plasmid.png

Sequencing

CYP2E1 sequencing.png

This is the sequencing result for the CYP2E1 human gene, codon optimized for S. cerevisiae expression. As can be seen, it is a perfect match to the expected sequence.

Application

CCA_SanDiego: We successfully incorporated the CYP2e1 gene alongside the required NADPH P450 reductase gene in a bicistronic construct for coexpression in bacteria. The aim is to use this construct for bioremediation purposes through expression in E. Coli.

T--CCA SanDiego--CYP2e1 Application.png