Difference between revisions of "Part:BBa K2100015"
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− | + | pDEST-mCherry is a destination vector which contains the mCherry gene, a monomeric fluorescent protein which emits red light upon excitation. The construct is used as a negative selection marker to recognize colonies of bacteria which have plasmids that did not successfully undergo the LR reaction and remain as destination vectors. | |
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Revision as of 02:40, 19 October 2016
pDEST_mCherry_GG
pDEST-mCherry is a destination vector which contains the mCherry gene, a monomeric fluorescent protein which emits red light upon excitation. The construct is used as a negative selection marker to recognize colonies of bacteria which have plasmids that did not successfully undergo the LR reaction and remain as destination vectors.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2466
Illegal XbaI site found at 2493
Illegal XbaI site found at 2797
Illegal SpeI site found at 2054
Illegal PstI site found at 1907
Illegal PstI site found at 2505 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2466
Illegal SpeI site found at 2054
Illegal PstI site found at 1907
Illegal PstI site found at 2505
Illegal NotI site found at 971 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2466
Illegal BamHI site found at 2487
Illegal XhoI site found at 1031 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2466
Illegal XbaI site found at 2493
Illegal XbaI site found at 2797
Illegal SpeI site found at 2054
Illegal PstI site found at 1907
Illegal PstI site found at 2505 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2466
Illegal XbaI site found at 2493
Illegal XbaI site found at 2797
Illegal SpeI site found at 2054
Illegal PstI site found at 1907
Illegal PstI site found at 2505
Illegal NgoMIV site found at 3360 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2749
Illegal BsaI site found at 4337
Illegal BsaI.rc site found at 2092