Difference between revisions of "Part:BBa K2150119"

 
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<h2>toxin ParE2 induced by IPTG</h2>
+
<h2>toxin YobR induced by IPTG</h2>
  
 
===Usage and Biology===
 
===Usage and Biology===
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===Characterization===
 
===Characterization===
 
<h4>Growth Curve</h4>
 
<h4>Growth Curve</h4>
We measured the growth curve of bacteria expressing toxin ParE2.
+
We measured the growth curve of bacteria expressing toxin YobR.
  
[[file:T--UCAS--6249t-IPTG.png|500px]]
+
[[file:T--UCAS--136t-IPTG.png|500px]]
  
 
The growth curve of wild type E. coli harboring empty vector is shown below.
 
The growth curve of wild type E. coli harboring empty vector is shown below.

Latest revision as of 01:41, 19 October 2016

toxin YobR induced by IPTG

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin YobR.

T--UCAS--136t-IPTG.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptac.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
  • 1000
    COMPATIBLE WITH RFC[1000]