Difference between revisions of "Part:BBa K1930006"

Revision as of 23:31, 18 October 2016

sfGFP(Sp)

sfGFP(Sp) is a reporter gene originally optimized for Streptococcus pneumoniae.

It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs). [http://aem.asm.org/content/79/20/6481.full]

Usage and Biology

Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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+Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.
 <span class='h3bb'>Sequence and Features</span>