Difference between revisions of "Part:BBa K1941002"

 
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<partinfo>BBa_K1941002 short</partinfo>
 
<partinfo>BBa_K1941002 short</partinfo>
  
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Scaffold RNA that targets TEF1 promoter and recruits two times the protein repressor module PCP-Mxi1 via the two effector protein recruitments sites PP7. PP7 is a well-characterized viral RNA sequence which is recognized by PCP protein. This RNA is composed of the dCas9-binding sgRNA which interacts with  Tef1, and two times the PCP-binding RNA hairpin PP7.
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Scaffold RNA (scRNA), with two times the PP7 hairpin, targeting TEF1 promoter.
  
  
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===Usage and Biology===
 
===Usage and Biology===
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A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding module. In this case, the hairpins are two times PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter TEF1.
  
 
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Latest revision as of 21:38, 18 October 2016


scTef1_2PP7

Scaffold RNA (scRNA), with two times the PP7 hairpin, targeting TEF1 promoter.


Usage and Biology

A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding module. In this case, the hairpins are two times PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter TEF1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]