Difference between revisions of "Part:BBa K1941000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Mxi1 was fused to PCP based on the fusion between VP64, a transcriptional activator, and MCP, another RNA binding module described in "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015. | |
| − | + | The link between PCP and Mxi1 is 6 base pairs long. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | PCP sequence taken from "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015 | |
===References=== | ===References=== | ||
Latest revision as of 20:35, 18 October 2016
PCP fused to Mxi1 domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
Illegal BglII site found at 333 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mxi1 was fused to PCP based on the fusion between VP64, a transcriptional activator, and MCP, another RNA binding module described in "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015. The link between PCP and Mxi1 is 6 base pairs long.
Source
PCP sequence taken from "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015