Difference between revisions of "Part:BBa K1923005"
 
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[1] Alexander, W. G., Doering, D. T., & Hittinger, C. T. (2014). High-efficiency genome editing and allele replacement in prototrophic and wild strains of saccharomyces. Genetics, 198(3), 859–866. http://doi.org/ 10.1534/genetics.114.170118
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[1] Alexander, W. G., Doering, D. T., & Hittinger, C. T. (2014). High-efficiency genome editing and allele replacement in prototrophic and wild strains of saccharomyces. Genetics, 198(3), 859–866.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:15, 18 October 2016


Thymidine kinase encoding gene

This basic part encodes an enzyme called thymidine kinase can function as a selectable and counterselectable marker gene in yeast. In certain medium condition TK positive yeast will survive while in another medium condition only TK negative yeast can grow [1].

For more information, please refer to the Wiki page of Team Tsinghua.

Reference:
[1] Alexander, W. G., Doering, D. T., & Hittinger, C. T. (2014). High-efficiency genome editing and allele replacement in prototrophic and wild strains of saccharomyces. Genetics, 198(3), 859–866.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]