Difference between revisions of "Part:BBa K1941000"
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<partinfo>BBa_K1941000 short</partinfo> | <partinfo>BBa_K1941000 short</partinfo> | ||
| − | + | RNA binding protein PCP fused to the transcriptional repression domain Mxi1. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | MAX-interacting protein 1 (Mxi1), is a transcriptional repressor of the c-Myc promoter. Initially found in mammalian cells, it is also active in yeast cells. PCP is a RNA binding protein that can be recruited by a small RNA sequence, PP7. | ||
| + | The fusion between these two proteins gives the possibility to Mxi1 to be recruited where the PP7 sequence is. For example, when producing PCP-Mxi1 along with BBa_K1941003, a scaffold RNA (SCRNA) with the PP7 sequence fused to it, the repressor is recruited to the scRNA and can repress CYC1 promoter. | ||
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Latest revision as of 20:08, 18 October 2016
PCP fused to Mxi1 domain
RNA binding protein PCP fused to the transcriptional repression domain Mxi1.
Usage and Biology
MAX-interacting protein 1 (Mxi1), is a transcriptional repressor of the c-Myc promoter. Initially found in mammalian cells, it is also active in yeast cells. PCP is a RNA binding protein that can be recruited by a small RNA sequence, PP7. The fusion between these two proteins gives the possibility to Mxi1 to be recruited where the PP7 sequence is. For example, when producing PCP-Mxi1 along with BBa_K1941003, a scaffold RNA (SCRNA) with the PP7 sequence fused to it, the repressor is recruited to the scRNA and can repress CYC1 promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
Illegal BglII site found at 333 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]