Difference between revisions of "Part:BBa K283010"

 
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__NOTOC__
 
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<partinfo>BBa_K283010 short</partinfo>
 
<partinfo>BBa_K283010 short</partinfo>
  
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This part codes for streptavidin which binds with biotin. The streptavidin coding region does not contain any rbs nor terminator
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This part codes for Streptavidin which binds with biotin. The Streptavidin coding region does not contain any rbs nor terminator.This part does not pose any biological threat.
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The UGent Belgium 2016 iGEM team improved this part by making it more practical for use in fusion proteins. The new part, [[Part:BBa_K1896000|BBa_K1896000]], codes for a monomeric Streptavidin mutant, contains an upstream peptide linker and is compatible with the RFC25 "Freiburg standard".
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:05, 18 October 2016

Streptavidin

This part codes for Streptavidin which binds with biotin. The Streptavidin coding region does not contain any rbs nor terminator.This part does not pose any biological threat.

The UGent Belgium 2016 iGEM team improved this part by making it more practical for use in fusion proteins. The new part, BBa_K1896000, codes for a monomeric Streptavidin mutant, contains an upstream peptide linker and is compatible with the RFC25 "Freiburg standard".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 85
    Illegal AgeI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]