Difference between revisions of "Part:BBa K1923002"

 
 
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<partinfo>BBa_K1923002 short</partinfo>
 
<partinfo>BBa_K1923002 short</partinfo>
  
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Reverse transcriptase is an enzyme.
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The msr-msd sequence in the part is flanked by two inverted repeats. Once transcribed, the msr-msd RNA folds into a secondary structure guided by the base pairing of the inverted repeats and the msr-msd sequence. The RT enzyme recognizes this secondary structure and uses a conserved guanosine residue in the msr as a priming site to reverse transcripe the msd sequence and produce a hybrid RNA-ssDNA molecule called msDNA. The two BsaI sites enable researchers to design ssDNA sequence produced by this cassette, thus providing a programmable in-vivo ssDNA expression system <sup>[1]</sup>.
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For more information, please refer to the Wiki page of Team Tsinghua.
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<strong>Reference:</strong>
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<br>
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[1] Farzadfard F, Lu T K. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations[J]. Science, 2014, 346(6211): 1256272.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:03, 18 October 2016


Scaffold msr-Scaffold msd-Reverse transcriptase encoding gene

The msr-msd sequence in the part is flanked by two inverted repeats. Once transcribed, the msr-msd RNA folds into a secondary structure guided by the base pairing of the inverted repeats and the msr-msd sequence. The RT enzyme recognizes this secondary structure and uses a conserved guanosine residue in the msr as a priming site to reverse transcripe the msd sequence and produce a hybrid RNA-ssDNA molecule called msDNA. The two BsaI sites enable researchers to design ssDNA sequence produced by this cassette, thus providing a programmable in-vivo ssDNA expression system [1].

For more information, please refer to the Wiki page of Team Tsinghua.

Reference:
[1] Farzadfard F, Lu T K. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations[J]. Science, 2014, 346(6211): 1256272.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 609
    Illegal XhoI site found at 363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 111
    Illegal BsaI.rc site found at 95