Difference between revisions of "Part:BBa K2075021:Design"

 
(→‎Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
 +
__NOTOC__
 +
<partinfo>BBa_K2075021 short</partinfo>
 +
 +
<partinfo>BBa_K2075021 SequenceAndFeatures</partinfo>
 +
 +
 +
===Design Notes===
 +
This part synthesize a transcription activator like (TAL) effector protein. They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion typ III system. In the plant cells these proteins binds promoter sites. This leads to a transcriptional activation of the genes behind the promoter. The proteins contains tandem repeats up to 34.5. Each repeat binds one nucleotide of the target sequenz. The 12th and 13th amino acid of each repeat leads to the specific binding of the DNA sequence.
 +
We circularised the TAL-effectors with the help of Inteins at the N- and C- Terminus of each TAL-effector.
 +
The 12th and 13th amino acid of each of the repeats from our TAL effector Ax7R-RR are: NG NN NI NI NN NN HD NG NG NN NI NG NN NI NN HD NG They bind the DNA sequence: T G/A A A G/A G/A C T T G/A A T G/A A G/A C T
 +
 +
===Source===
 +
 +
Source info goes here
 +
 +
===References===
 +
References go here

Latest revision as of 19:27, 18 October 2016


TAL-effector Ax7R-RR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2939
    Illegal BamHI site found at 2345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2284
    Illegal NgoMIV site found at 2879
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2359


Design Notes

This part synthesize a transcription activator like (TAL) effector protein. They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion typ III system. In the plant cells these proteins binds promoter sites. This leads to a transcriptional activation of the genes behind the promoter. The proteins contains tandem repeats up to 34.5. Each repeat binds one nucleotide of the target sequenz. The 12th and 13th amino acid of each repeat leads to the specific binding of the DNA sequence. We circularised the TAL-effectors with the help of Inteins at the N- and C- Terminus of each TAL-effector. The 12th and 13th amino acid of each of the repeats from our TAL effector Ax7R-RR are: NG NN NI NI NN NN HD NG NG NN NI NG NN NI NN HD NG They bind the DNA sequence: T G/A A A G/A G/A C T T G/A A T G/A A G/A C T

Source

Source info goes here

References

References go here