Difference between revisions of "Part:BBa K2097001"
 
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<partinfo>BBa_K2097001 short</partinfo>
 
<partinfo>BBa_K2097001 short</partinfo>
  
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This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B003.
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This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002  </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>.
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===Usage and Biology===
 
===Usage and Biology===
  
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BBa_K592009 was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. BBa_K608002 was created by Freiburg 2011.
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<a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. <a href="http://partsregistry.org/Part:BBa_K608002">BBa_K608002</a> was created by Freiburg 2011.
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[[File:T--Austin_UTexas--BCPplate.png|thumb|left|300px| Figure 1. amilCP expressed in <i> E. coli </i> and on an LB/agar plate ]]
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[[File:T--Austin_UTexas--BCPtube.png|thumb|center|150px| Figure 2. amilCP expressed in <i> E. coli </i> and in liquid LB]]
  
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[[File:T--Austin_UTexas--BCPplate.png|thumb|left|600px| Figure 3. Control amilCP.  This control was used to compare the color intensity to the pH sensitive P-atp2 while testing with a range of pH values. This part was documented to not be affected by changes in pH.]]
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K2097001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2097001 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:41, 18 October 2016


Blue chromoprotein with strong promoter and RBS

This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034.

Usage and Biology

BBa_K592009 was created by Uppsala-Sweden 2011. It comes from the coral Acropora millepora, amilCP. BBa_K608002 was created by Freiburg 2011.

Figure 1. amilCP expressed in E. coli and on an LB/agar plate
Figure 2. amilCP expressed in E. coli and in liquid LB


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]